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Ludwig Boltzmann Institute for Experimental and Clinical Traumatolgy, Austrian Cluster for Tissue Regeneration, Laser Consult Austria Low level pulsed light LED light therapy of different wavelengths modulates vasculogenesis
Conclusion
Light therapy with green or red light regulates different mechanism within endothelial cells which are involved in vasculogenesis. In a 2D culture model, one time illumination
led to an increase in proliferation, migration and immediate nitric oxide (NO) levels, crucial processes and components required for vessel formation during regeneration.
Continuous illumination of endothelial cells with green or red light for 7 days in a 3D model enhanced migration. Pro-angiogenic (HGF, NRG1-b1, PIGF, CXCL16 & DPPIV) and
anti-angiogenic (serpin F1) proteins were expressed post-illumination, which suggests an interplay of pro- and anti-angiogenic factors during remodeling and vasculogenesis.
In a co-culture model of endothelial cells and adipose-derived stem cells at a 1:0.01 ratio, no network formation was detected, but illumination with green or red light enhanced
precursors of vascular tube formation, namely stretching and elongation of cells. This data suggests that LLLT has a positive effect on the induction of vasculogenesis and
could facilitate vessel formation as it is easily applicable and a cost-effective therapy.
Study:
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