Burn wounds are a common challenge for medical professionals. Current burn wound models hold
several limitations, including a lack of comparability due to the heterogeneity of wounds and
differences in individual wound healing. Hence, there is a need for reproducible in vivo models. In this
study, we established a new burn wound model using the chorioallantoic membrane assay (CAM) as
a surrogate model for animal experiments. The new experimental setup was tested by investigating
the effects of the auspicious biophysical therapy, photobiomodulation (PBM), on the wound healing
of an induced CAM burn wound with a metal stamp. PBM has been shown to positively influence
wound healing through vascular proliferative effects and the increased secretion of chemotactic
substances. The easily accessible burn wounds can be treated with various therapies. The model
enables the analysis of ingrowing blood vessels (angiogenesis) and diameter and area of the wounds.
The established model was used to test the effects of PBM on burn wound healing. PBM promoted
angiogenesis in burn wounds on day 4 (p = 0.005). Furthermore, there was a not significant trend
toward a higher number of vessels for day 6 (p = 0.065) in the irradiated group. Changes in diameter
(p = 0.129) and the burn area (p = 0.131) were not significant. Our results suggest that CAM can be a
suitable model for studying burn wounds. The novel experimental design enables reproducible and
comparable studies on burn wound treatment.
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