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Testing the effectsof photobiomodulationon angiogenesis in a newlyestablished CAM burn woundmodel

Burn wounds are a common challenge for medical professionals. Current burn wound models hold

several limitations, including a lack of comparability due to the heterogeneity of wounds and

differences in individual wound healing. Hence, there is a need for reproducible in vivo models. In this

study, we established a new burn wound model using the chorioallantoic membrane assay (CAM) as

a surrogate model for animal experiments. The new experimental setup was tested by investigating

the effects of the auspicious biophysical therapy, photobiomodulation (PBM), on the wound healing

of an induced CAM burn wound with a metal stamp. PBM has been shown to positively influence

wound healing through vascular proliferative effects and the increased secretion of chemotactic

substances. The easily accessible burn wounds can be treated with various therapies. The model

enables the analysis of ingrowing blood vessels (angiogenesis) and diameter and area of the wounds.

The established model was used to test the effects of PBM on burn wound healing. PBM promoted

angiogenesis in burn wounds on day 4 (p = 0.005). Furthermore, there was a not significant trend

toward a higher number of vessels for day 6 (p = 0.065) in the irradiated group. Changes in diameter

(p = 0.129) and the burn area (p = 0.131) were not significant. Our results suggest that CAM can be a

suitable model for studying burn wounds. The novel experimental design enables reproducible and

comparable studies on burn wound treatment.


s41598-023-50165-6 (6)
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